Asia Journal of Public Health : Volume 5 No. 0, December 2014. 16-20
Discrimination of Plasmodium falciparum Multidrug-resistant 1 allele Carried by 3D7 Using Loop-mediated Isothermal Amplification Method
Usa Lek-Uthai* Siriporn Rachakhom**
ABSTRACT: A recent survey found drug-resistant malaria along Thai-Myanmar and Thai-Cambodian border provinces. Molecular markers for drug-resistant malaria, such as Plasmodium falciparum mdr1 3D7 polymorphisms, could serve as a useful surveillance tool. The proper technologies for the detection of markers should be modified. The discrimination of 3D7Pfmdr1 gene from filter blood spots using Loop-mediated isothermal amplification (LAMP) method was purposed while Real-time PCR was a confirmed method. In this study, 80.55% (58/72) 3D7Pfmdr1 using LAMP method was discriminated. Real-time PCR was a confirmed method, which had a result of 86.11% (62/72). The gDNA concentrations of four false negatives cases (4/62) for 3D7Pfmdr1 which were less than 0.13ng/µl, could not be detected by LAMP. However, the LAMP method has the advantages of simplicity of reaction in room temperature, does not require any advanced equipment, has a low cost, and is an early and rapid discrimination method and these reasons will provide a public health tool to detect multidrug resistance allele efficiently at the field site.
*Department of Parasitology and Entomology, Faculty of Public Health, Mahidol University, Bangkok, Thailand
** Infectious Diseases and Epidemiology Program, Faculty of Graduate Studies, Mahidol University, Na Khon Pa Thom, Thailand